The effect of humic acid on rapeseed (Brassica napus L.) plant growth, heavy metal uptake, phytoremediation parameters (BCF, TF and TI), and antioxidant activity in heavy metal polluted soil

The aim of this study was to investigate the effects humic acid (HA) applications on rapeseed (Brassica napus L.) growth, heavy metal uptake, bioconcentration factor (BCF), translocation (TF), tolerance index (TI), catalase (CAT), ascorbate peroxidase (APX) enzyme activities and hydrogen peroxide (H2O2) content in polluted soil with lead (Pb), chromium (Cr), cadmium (Cd), zinc (Zn). Three doses HA (Control, HA1:500 mg kg-1, HA2:1000 HA3:2000 kg-1) were applied pots. HA1, HA2, HA3 increased plant growth parameters compared soil. Compared control, Pb, Cr, Cd, Zn concentrations plant. However, significantly decreased roots shoots BCF both plants greater than 1 for Zn. specifically HA2 application shoot root values TF smaller On other hand, Cd values. Shoot TI 17.37 %, 9.09% CAT APX H2O2 rapeseed. It is concluded that has a remedial effect development by reducing oxidative stress.